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Teachers

If you'd like to carry out these experiments with your middle school students, request reagents here

Growing banana-scented bacteria

Check out how cells grow and why isoamyl OH must be added to the media

Before you begin your work you should put on personal protective equipment as deemed appropriate by your school. These may include

  • nitrile gloves
  • eye protection and
  • a lab coat.

lab protocol

  1. Label a 15 ml screw-capped tube “Transformed Cells”
  2. Transfer 3 ml of Luria Broth (LB) + ampicillin + isoamyl alcohol to the tube.
  3. Using a pipet tip, scoop up a bacterial colony from the pBNAN petri dish and throw the tip into the media in the tube labelled “Transformed Cells.”
  4. Label a second 15 ml screw-capped tube “Starting Cells.”
  5. Transfer 3 ml of Luria Broth (LB) + isoamyl alcohol (NO AMPICILLIN) to the tube.
  6. Using a pipet tip, transfer a bit of bacteria from the “NB370” petri dish and throw the tip into the media in the tube labelled “Starting Cells.”
  7. Place the culture tubes on a shaker platform (?) in the incubator at 37°C (or Room Temp?) overnight.


Need to write up smell test day.

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